Identification of donor hematopoietic progenitor cells after allogeneic liver transplantation.
نویسندگان
چکیده
Inbred male Lewis (LEW) and Brown Norway (BN) rats were used for experiments. Plastic dish nonadherent bone marrow cells (BMC) were prepared and cultured with 2% PWM-SCM for 6 days in a fibrin clot culture system to detect colony-forming units in culture (CFU-C). Clusters (10 to 50 cells per aggregate) and colonies (>50 cells per aggregate) in cultures were counted as CFU-C. LEW-derived CFU-C was identified by in situ immuno· histochemical staining of cultured cells in the fibrin clot, using a standard three-step avidin-biotin complex method with monoclonal antibody L21-6. which recognizes the class II MHC antigens of LEW but not BN rats.
منابع مشابه
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عنوان ژورنال:
- Transplantation proceedings
دوره 29 1-2 شماره
صفحات -
تاریخ انتشار 1997